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Defining the Role of Estrogen Receptor β in the Regulation of Female Fertility.

Endocrinology. 2017; 
Rumi MAK,, Singh P,, Roby KF,, Zhao X,, Iqbal K,, Ratri A,, Lei T,, Cui W,,, Borosha S,, Dhakal P,, Kubota K,, Chakraborty D,, Vivian JL,, Wolfe MW,, Soares MJ,,.
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Monoclonal Antibody Services Flag-tagged proteins were detected using a mouse monoclonal anti-FLAG antibody (A00187; Research Resource Identifier: AB_1720813; GenScript, Piscataway, NJ).... EMD Millipore, 05-824 Produced in collaboration with Epitomics Species Raised in; Monoclonal or Polyclonal Rabbit; monoclonal Dilution Used 5000 RRID AB_11212759 Mouse anti-DYKDDDDK- tag mAb; mouse anti- flag-tag mAb; Anti-b-actin antibody, mouse; monoclonal GenScript, A00187-200 Mouse; monoclonal 5000 AB_1720813 Sigma-Aldrich, A 1978-100 UL Mouse; monoclonal 25,000 AB_476692 Abbreviations: mAB, monoclonal antibody; MS, manuscript; RRID, Research Resource Identifier. Get A Quote

摘要

Estrogens are essential hormones for the regulation of fertility. Cellular responses to estrogens are mediated by estrogen receptor α (ESR1) and estrogen receptor β (ESR2). In mouse and rat models, disruption of Esr1 causes infertility in both males and females. However, the role of ESR2 in reproductive function remains undecided because of a wide variation in phenotypic observations among Esr2-mutant mouse strains. Regulatory pathways independent of ESR2 binding to its cognate DNA response element have also been implicated in ESR2 signaling. To clarify the regulatory roles of ESR2, we generated two mutant rat models: one with a null mutation (exon 3 deletion, Esr2ΔE3) and the other with an inframe deletion ... More

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