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Von Willebrand Factor Type A domain of hCLCA1 is sufficient for U-937 macrophage activation.

Biochem Biophys Rep. 2019; 
KeithBrandon A,ChingJohn C H,LoewenMatth
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摘要

The human hCLCA1 gene is a member of the gene family that has a well-documented role in inflammatory airway diseases. Previously, we demonstrated that secreted hCLCA1 plays a role in regulating the innate immune response by activating airway macrophages. However, the mechanism of this regulation remains unclear. In this present study, recombinant proteins containing different hCLCA1 domains are expressed to determine the specific hCLCA1 domain(s) responsible for macrophage activation. Specifically, hCLCA1 constructs containing the hydrolase domain (HYD), the von Willebrand Factor Type A (VWA) domain, and the fibronectin type III (FN3) domain were heterologously expressed and affinity purified throu... More

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