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Removal Endotoxin From rAAV Samples Using Simple Detergent-based Protocol

Molecular Therapy. 2019-09; 
Liudmyla Kondratova, Oleksandr Kondratov, Ragy Ragheb, Sergei Zolotukhin
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Cellular Analysis LPS levels were detected using Limulus Amebocyte Lysate assay (GenScript, L00350) with 0.1 – 1 EU/ml linear range. Kits of separate lots have distinct calibration curves that could be different from one lot to another Get A Quote

摘要

Endotoxin is the most common contaminant found in protein samples. Even small amount of endotoxin can induce strong allergic reaction and death of a host organism. Endotoxin is also often detected in recombinant Adeno-associated virus (rAAV) stocks prepared in research laboratories using off-the-shelf reagents; purifying rAAV stocks from endotoxin using commercial reagents sometimes results in significant titer loss. The problem is exacerbated due to recently expanded diversity of rAAV serotypes and capsid variants which, due to their variable capsid surface charge, display differential affinity towards endotoxin. In this paper, we describe a simple universal protocol of purifying vector stocks irrespective of ... More

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