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Endothelial cells promote 3D invasion of GBM by IL-8-dependent induction of cancer stem cell properties.

Sci Rep. 2019-06; 
McCoyMichael G,NyanyoDennis,HungCarol K,GoergerJulian Palacios,R ZipfelWarren,WilliamsRebecca M,NishimuraNozomi,FischbachCla
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Recombinant Proteins while recombinant IL-8 (50?ng/mL, GenScript) was used for IL-8 supplementation. Get A Quote

摘要

Rapid growth and perivascular invasion are hallmarks of glioblastoma (GBM) that have been attributed to the presence of cancer stem-like cells (CSCs) and their association with the perivascular niche. However, the mechanisms by which the perivascular niche regulates GBM invasion and CSCs remain poorly understood due in part to a lack of relevant model systems. To simulate perivascular niche conditions and analyze consequential changes of GBM growth and invasion, patient-derived GBM spheroids were co-cultured with brain endothelial cells (ECs) in microfabricated collagen gels. Integrating these systems with 3D imaging and biochemical assays revealed that ECs increase GBM invasiveness and growth through inter... More

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