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Differential Immunoreactivity to Bovine Convalescent Serum Between Mycoplasma bovis Biofilms and Planktonic Cells Revealed by Comparative Immunoproteomic Analysis
Therecombinant proteins were expressed inE. coliBL21(DE3)cells (Novagen, Darmstadt, Germany), purified by NTA-NI(Genscript, Nanjing, China), and examined by SDS-PAGE.