Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs
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… TrCel7B and TrCel6A from T. reesei were purified as described by Suurn?kki et al. [28]. The gene
encoding T. aurantiacus LPMO9A (also known as TaGH61A; [11]) including its native signal
sequence was codon optimized for Pichia pastoris (GenScript, Piscataway, NJ, USA) … |
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Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass， especially the discovery of lytic polysaccharide monooxygenases (LPMOs)， have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world， including Scandinavia， but efficient pretreatment and subsequent enzymatic hydrolysis of softwoods are challenging. Sulfite pulping-based pretreatments， such as in the BALI? process， yield substrates that are relatively easy to degrade. We have assessed how process conditions affect the efficiency of modern cellulase preparations in processing of such substrates.
Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass， especially the discovery of lytic polysaccharide monooxygenases (LPMOs)， have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world， including Scandinavia， but efficient pretreatment and subsequent enzymatic hydrolysis of softwoods are challenging. Sulfite pulping-based pretreatments， such as in the BALI? process， yield substrates that are relatively easy to degrade. We have assessed how process conditions affect the efficiency of modern cellulase preparations in processing of such substrates.
AA9，Biofuel，Cellulase，GH61，LPMO，Lignocellulose，Pretreatment，Pulping，Sul
