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Characterization of a leukocidin identified in Staphylococcus pseudintermedius.

PLoS ONE. 2018-09; 
AbouelkhairMohamed A,BemisDavid A,GiannoneRichard J,FrankLinda A,KaniaSteph
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Proteins, Expression, Isolation and Analysis … Recombinant attenuated LukS-I and LukF-I produced in E.coli were purified using affinity chromatography (as above) and endotoxin concentrations were measured using a chromogenic LAL Endotoxin Assay Kit (Genscript, Cat. No. L00350) … Get A Quote

摘要

Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair the innate immune system, the first line of defense against these pathogens. Seven different leukocidins have been characterized in Staphylococcus aureus, some of which are host and cell specific. Through genome sequencing and analysis of the S. pseudintermedius secretome using liquid chromatography mass spectrometry we identified two proteins, named "LukS-I" and "LukF-I", encoded on a degenerate prophage contained in the ... More

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