Species |
SARS-CoV-2 |
Protein Construction |
S1 protein (Gln14-Arg685) Accession # P0DTC2 |
|
Purity |
> 90% as analyzed by SDS-PAGE |
Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
Biological Activity |
SARS-CoV-2 Spike protein (S1) can bind with Human ACE2 in functional ELISA assay. |
Expression System |
293 Cells |
Theoretical Molecular Weight |
78.3 kDa (Additional amino
acids from the vector are included. The sequence of them is confidential.) |
Formulation |
Supplied as a solution in PBS pH 7.4 |
Concentration |
Please refer to the COA for the specific lot. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
Western blot analysis of SARS-CoV-2 Spike protein (S1)
(GenScript, Z03501) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03501
Lane 2: 5ng of Z03501
Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No.?Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No.?Z03484) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).
Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
> 85% as analyzed by SDS-PAGE
Target Background |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity. |
Synonyms |
SARS-CoV-2 S1 protein; 2019-nCoV S1 protein |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.