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Fig. A-1 (left figure): Inclusion body preparation and denaturation
Lane 1: Total protein after sonication
Lane 2: The supernatant after centrifugation
Lane 3: Washed with 1 M urea
Lane 4: Dissolved by 8 M urea
Fig. A-2 (right figure): Refolded protein
Lane 1: Refolded tag-free A protein
Fig. B-1 (left figure): Inclusion body preparation and denaturation
Lane 1: The supernatant after centrifugation
Lane 2: The precipitation after centrifugation
Lane 3: Inclusion bodies dissolved by urea
Lane 4: Flow through
Lane 5: Washed by imidazole containing urea
Lane 6: Eluted by imidazole containing urea
Fig. B-2 (right figure): Refolded protein
Lane 1 and 2: Refolded B fusion protein
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Kansas State University, USA
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