| Specificity |
GenScript MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66), mAb, Rabbit is specific for SARS-CoV-2 Nucleocapsid protein of wild type, delta variant, and omicron variant. |
| Host Species |
Rabbit |
| Immunogen |
Recombinant SARS-CoV-2 Nucleocapsid protein |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
1:1,000 - 1:5,000 |
| Chemiluminescent immunoassay (CLIA) |
1:1,000 - 1:5,000 |
| Western Blot |
1:100 - 1:1,000 |
|
| Form |
Liquid |
| Storage Buffer |
PBS, pH 7.2, 0.02% sodium azide |
| Concentration |
1 mg/ml |
| Storage Instructions |
Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
N66 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
Western Blot of wild-type and delta variant Nucleocapsid protein cell lysates with MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66), mAb, Rabbit (GenScript, A02137).
M: Protein Marker (GenScript, M00521)
Lane 1: 3 μg Wild-type N protein cell lysate
Lane 2: 3 μg Delta N protein cell lysate
Primary Antibody:
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66), mAb, Rabbit (GenScript, A02137) 1 μg/ml
Secondary Antibody:
Goat Anti-Rabbit IgG-HRP (GenScript, A00131)
BLI (Biolayer interferometry) binding affinity measurements of MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66), mAb, Rabbit (GenScript, A02137) to SARS-CoV-2 Nucleocapsid protein of wild type and delta variant.
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66) captured on Protein A sensors surface can bind wild-type or delta variant with dissociation constant (KD) of 5.95E-10M and 2.75E-09M.
BLI (Biolayer interferometry) binding affinity measurements of MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66), mAb, Rabbit (GenScript, A02137) to nucleocapsid protein of SARS-CoV-2 omicron variant.
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N66) captured on Protein A sensors surface can bind omicron variant with dissociation constant (KD) of 6.41E-10M.
| Target Background |
SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). Nucleocapsid Protein is a most abundant structure protein of the coronavirus which is associated with nucleic acid. |
| Synonyms |
Rabbit Anti-COVID19 N Protein Antibody; Rabbit Anti-SARS-CoV-2 NP Antibody; Sars/sars-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
