| Specificity |
GenScript Protein A Antibody [Biotin], mAb, Mouse detects natural Protein A, variety of recombinant Protein A variants and MabSelect SuReTM ligand. |
| Host Species |
Mouse |
| Immunogen |
purified recombinant Protein A |
| Conjugate |
Biotin |
|
Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Sandwich ELISA |
Capture with A01778 and detected by A01779
Capture: 1-10 μg/ml
Detection: 0.05-0.2 μg/ml |
| Western Blot |
0.5 -1 μg/ml |
|
| Form |
lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
5G10A7 |
| Target Background |
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The Protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of Protein A in sample foods or for evaluation of Protein A resin. This Protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to Protein A.GenScript Protein A Antibody [Biotin], mAb, Mouse is Protein A Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of Protein A. |
| Synonyms |
Protein A Antibody [Biotin], mAb, Mouse; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
