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Identification and application of an exocarp-preferential promoter for genetic engineering of tomato fruit

Hortic Res. 2024-01; 
Xue-Ming Ruan, Xiangyu Xiong, Jian-Feng Li
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摘要

Tomato () is a globally cultivated crop with great economic value. The exocarp determines the appearance of tomato fruit and protects it from various biotic and abiotic challenges at both pre-harvest and post-harvest stages. However, no tomato exocarp-specific promoter is currently available, which hinders exocarp-based genetic engineering. Here, we identified by RNA sequencing and reverse transcription-quantitative PCR analyses that the tomato gene () was abundantly and predominantly expressed in the exocarp. A fluorescent reporter expressed by a 2087-bp promoter () was mainly detected in the exocarp of transgenic tomato plants of both Ailsa Craig and Micro-Tom cultivars. This promoter was further utilized f... More

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