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Recombinant adenovirus expressing the fusion protein PD1PVR improves CD8 T cell-mediated antitumor efficacy with long-term tumor-specific immune surveillance in hepatocellular carcinoma

Cell Oncol (Dordr). 2021-09; 
Hailin Zhang, Yonghui Zhang, Jie Dong, Shuguang Zuo, Gang Meng, Junhua Wu, Jiwu Wei
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Catalog Antibody … Then, the membranes were incubated with a specific primary mouse anti-his antibody (Cat# A00186, GenScript Biotech, Nanjing, China) at 4 ℃ overnight?… Get A Quote

摘要

objective: Treatment-associated upregulation of suppressive checkpoints and a lack of costimulatory signals compromise the antitumor efficacy of oncolytic virus immunotherapy. Therefore, we aimed to identify highly effective therapeutic targets to provide a proof-of-principle for immune checkpoint together with oncolytic virus-mediated viro-immunotherapy for cancer. methods: A fusion protein containing both the extracellular domain of programmed death-1 (PD-1) and the poliovirus receptor (PVR) was designed. Next, the corresponding expression fragment was inserted into the genome of a replication-competent adenovirus to generate Ad5sPD1PVR. The infection, expression, replication and oncolysis of Ad5sPD1PVR were ... More

關鍵詞

Fludarabine, Hepatocellular carcinoma, Oncolytic virus, PD-1/PD-L1, PVR/CD155, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT)
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