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A Novel Ig Domain-Containing C-Type Lectin Triggers the Intestine-Hemocyte Axis to Regulate Antibacterial Immunity in Crab

J Immunol. 2022-05; 
Kaimin Zhou, Yukai Qin, Yu Song, Ke Zhao, Weijuan Pan, Xingyu Nan, Yue Wang, Qun Wang, Weiwei Li
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Gene Synthesis … (L00210; GenScript, … were injected with the target gene dsRNA or dsGFP control, respectively. After EsIgLectin or EspIgR was knocked down by dsRNA injection, all crabs were injected … Get A Quote

摘要

The C-type lectin family with the signature C-type lectin-like domain promotes antibacterial host defense within the animal kingdom. We examined the role of Chinese mitten crab, (H. Milne-Edwards) (Decapoda: Grapsidae) Ig domain-containing C-type lectin (IgLectin), a novel and poorly understood member of the C-type lectin family. was expressed primarily by both hemocytes ( ) and intestines, with significantly induced mRNA expression on intestinal or hemolymph bacterial infections. As a soluble protein, both its C-type lectin-like domain and the Ig domain were required for bacterial binding, bacterial agglutination, bacterial growth inhibition, and in vivo bacterial clearance. Polymeric IgLectin could be const... More

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