Plant growth-promoting rhizobacteria (PGPR) and their extracellular polymers such as exopolysaccharides can enhance rice salt stress resistance, however, the relevant mechanism remains unclear. In this study, an exopolysaccharides-deficient strain, named ΔpspD, was obtained from Pantoea alhagi NX-11 by chromosomal pspD deletion. The yield and characteristics of ΔpspD exopolysaccharides was obviously different from P. alhagi NX-11 exopolysaccharides (PAPS). Subsequently, hydroponic experiments showed that NX-11 or PAPS could enhance rice salt tolerance, but ΔpspD could not. Furthermore, it was found that PAPS promoted P. alhagi rhizosphere colonization through a direct effect on biofilm formation, as well as through an indirect impact of enhancing the abilities of biofilm formation and chemotaxis by altering rice root exudates. Importantly, the effect of PAPS in promoting the root colonization of NX-11 was specific. Through transcriptome and RT-qPCR analysis, we revealed that this specificity correlated with PAPS-induced lectin overexpression. The specificity between exopolysaccharides and the host microorganism ensures the colonization of the latter, and prevents other microorganisms from hitchhiking to the rice roots.