Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host-parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins' expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish's immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium , and in a second trial, fish were exposed to the ectoparasite and subsequently infected with the intracellular bacterium . Fish immunized with IPath showed a significant delay in the mortality curve in response to and infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high modulation of iron-homeostasis-related genes in fish vaccinated with IPath compared to the control group during the infection. Moreover, increasing expression of Atlantic salmon was associated with IPath immunization. This study provides evidence that the IPath protein could be used as an antigen or booster in commercial fish vaccines, improving the immune response against relevant pathogens for salmon aquaculture.