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Pathogenicity of porcine deltacoronavirus (PDCoV) strain NH and immunization of pregnant sows with an inactivated PDCoV vaccine protects 5-day-old neonatal piglets from virulent challenge

Transbound Emerg Dis. 2019-09-01; 
Jialin Zhang, Jianfei Chen, Ye Liu, Shi Da, Hongyan Shi, Xin Zhang, Jianbo Liu, Liyan Cao, Xiangdong Zhu, Xiaobo Wang, Zhaoyang Ji, Li Feng
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Recombinant Antibody Expression … Recombinant S protein was expressed in 293T cells, purified using anti-DYKDDDDK G1 Affinity Resin (GenScript: L00432-1), and used as the … swine coronaviruses. Vet Microbiol, 206, 45-51 … immunity to porcine respiratory coronavirus to protection against transmissible … Get A Quote

摘要

In this study, the pathogenicity of porcine deltacoronavirus (PDCoV) strain NH (passage 10, P10) was evaluated. We found that PDCoV strain NH is enteropathogenic in 5-day-old pigs. Pathogenicity experiments provided a challenge model for studying the protection efficiency of passive immunity. In order to investigate the protective efficacy of passive immunity in newborn piglets, pregnant sows were vaccinated with either a PDCoV-inactivated vaccine at the Houhai acupoint (n?=?5) or DMEM as a negative control (n?=?2) using a prime/boost strategy 20 and 40?days before delivery. PDCoV spike (S)-specific IgG and neutralizing antibody (NA) responses were detected in immunized sows and piglets born to immunized s... More

關鍵詞

inactivated PDCoV vaccine, passive immunity, pathogenicity, piglets, sows
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