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Minimal system for assembly of SARS-CoV-2 virus like particles

biorxiv. 2020-06;

Heather?Swann,?Abhimanyu?Sharma,?Benjamin?Preece,?Abby?Peterson,?Crystal?Eldridge,?David M.?Belnap,?ProfileMichael?Vershinin,?ProfileSaveez?Saffarian

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Recombinant Proteins	When the 293T cells were at 50% confluent in the 100 mm dishes, they were co-transfected with 29.5 μg of humanized membrane in pCDNA3.1, 5.9 μg of humanized spike in pCDNA3.1, and 5.9 μg of humanized envelope in pCDNA3.1 (GenScript).?	Get A Quote

摘要

SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs display numerous S protein spikes ideal for vaccine development. The particles have a spike to spike size of 103 ± 6 nm and a membrane diameter of 63 ± 5 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.