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Cellular Analysis> | … PK activity was detected using a PK Assay Kit (Jiancheng Technology Co Ltd, Nanjing, China) … Precast gels of 10% (M00664, Genscript, USA) were used to analyze total content and S-nitrosylated content of GP, PFK and PK … | Get A Quote |
The activity, expression and S-nitrosylation of glycogen phosphorylase (GP), phosphofructokinase (PFK) and pyruvate kinase (PK) was compared between pale, soft and exudative (PSE) and red, firm and non-exudative (RFN) pork. The nitric oxide synthase (NOS) activity of RFN pork was higher than PSE pork (P?0.05). Glycogen and lactic acid content were significantly different between PSE and RFN samples at 1?h postmortem (P?0.05). Compared to PSE pork, RFN pork had lower activities and higher S-nitrosylation levels of GP, PFK and PK (P?0.05). Moreover, GP expression in RFN pork was lower (P?0.05) while no significant differences of PFK and PK expression were observed between these two groups. These ... More