| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | E12, Cell Signaling Technology, Danver, MA, USA), ATM (2C1, Santa Cruz, CA, USA), p-T1989-ATR (GTX128145, GeneTex, Irvine, CA, USA), ATR (sc28901, Santa Cruz), pS824-Kap1 (Bethyl Laboratories, Montgomery, TX, USA), Kap1 (Bethyl Laboratories), p-S317-Chk1 (2344, Cell Signaling Technology), Chk1 (sc8408, Santa Cruz), p-S966-Smc1 (A300-050 A, Bethyl Laboratories), Smc1 (ab9262, AbCam), p53 (DO-1, Santa Cruz), pS15-p53 (Cell Signaling Technology), pT68-Chk2 (Cell Signaling Technology), Chk2 (Clone 7, EMD-Millipore), pS33-RPA (A300-246 A, Bethyl Laboratories), RPA (sc56770, Santa Cruz), Ub-PCNA (Lys 164) (13439, Cell Signaling Technology), PCNA (2586, Cell Signaling Technology), PCNA-HRP (Clone PC10, sc56 Santa Cruz), His (A00186-100, Genscript, Piscataway, NJ, USA), Flag M2 (Sigma-Aldrich), c-Myc (Sigma- Aldrich), β-actin (Sigma-Aldrich), alpha-tubulin (ab7291, AbCam), GAPDH-HRP (ab9385, Abcam) and HRP-conjugated goat anti-mouse/ rabbit IgG (Sigma-Aldr ich). | Get A Quote |
The DNA replication machinery invariably encounters obstacles that slow replication fork progression, and threaten to prevent complete replication and faithful segregation of sister chromatids. The resulting replication stress activates ATR, the major kinase involved in resolving impaired DNA replication. In addition, replication stress also activates the related kinase ATM, which is required to prevent mitotic segregation errors. However, the molecular mechanism of ATM activation by replication stress is not defined. Here, we show that monoubiquitinated Proliferating Cell Nuclear Antigen (PCNA), a marker of stalled replication forks, interacts with the ATM cofactor ATMIN via WRN-interacting protein 1 (WRNIP1).... More
