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Development of enzyme-linked immunosorbent assays using 2 truncated ORF2 proteins for detection of IgG antibodies against hepatitis E virus.

Ann Lab Med. 2014;

TaherkhaniReza，MakvandiManoochehr，F(xiàn)arshadpourFat

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Codon Optimization	… The nucleotide sequence of the truncated ORF2 gene (ORF2.1, encoding amino acids 112-660) of HEV genotype 1, isolate sar55 (Gen-Bank accession number AF444002.1) was analyzed using GenScript Rare Codon Analysis Tool (GenScript USA Inc., Piscataway, NJ, USA) …	Get A Quote

摘要

Without appropriate culture systems for hepatitis E virus (HEV)， sufficient natural viral proteins are difficult to generate for use in serological tests. Therefore， it is important to produce large amounts of HEV recombinant proteins in an economical way. The present study developed ELISAs using 2 truncated forms of the HEV open reading frame (ORF) 2 protein in order to detect anti-HEV IgG in serum samples.