Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine
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| Codon Optimization |
… codon-optimized for protein expression in swine. Synthesis, codon-optimization,
cloning in pUC57 vector, and sequence-verification of these genes was outsourced
(GenScript, NJ, USA). Each target gene was then amplified … |
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African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. Lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. In this study， we evaluated the immunogenicity of seven adenovirus-vectored novel ASFV antigens， namely A151R， B119L， B602L， EP402RΔPRR， B438L， K205R and A104R. Immunization of commercial swine with a cocktail of the recombinant adenoviruses formulated in adjuvant primed strong ASFV antigen-specific IgG responses that underwent rapid recall upon boost. Notably， most vaccinees moun... More
African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. Lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. In this study， we evaluated the immunogenicity of seven adenovirus-vectored novel ASFV antigens， namely A151R， B119L， B602L， EP402RΔPRR， B438L， K205R and A104R. Immunization of commercial swine with a cocktail of the recombinant adenoviruses formulated in adjuvant primed strong ASFV antigen-specific IgG responses that underwent rapid recall upon boost. Notably， most vaccinees mounted robust IgG responses against all the antigens in the cocktail. Most importantly and relevant to vaccine development， the induced antibodies recognized viral proteins from Georgia 2007/1 ASFV-infected cells by IFA and by western blot analysis. The recombinant adenovirus cocktail also induced ASFV-specific IFN-γ-secreting cells that were recalled upon boosting. Evaluation of local and systemic effects of the recombinant adenovirus cocktail post-priming and post-boosting in the immunized animals showed that the immunogen was well tolerated and no serious negative effects were observed. Taken together， these outcomes showed that the adenovirus-vectored novel ASFV antigen cocktail was capable of safely inducing strong antibody and IFN-γ+ cell responses in commercial swine. The data will be used for selection of antigens for inclusion in a multi-antigen prototype vaccine to be evaluated for protective efficacy.
