Parenteral nutrition (PN) is associated with increased infectious risks due to impaired intestinal immunity. Although glucagon-like peptide-2 (GLP-2) enhances the gut barrier function， it is uncertain whether it improves mucosal immunologic barrier function. We hypothesized that injecting the PN mouse model with GLP-2 improved innate and acquired immunity， and prevented bacterial translocation. Forty-eight hours after venous cannulation， male Institute of Cancer Research mice were randomly divided into 3 groups based on their diet: chow with saline (n = 10)， PN (n = 9)， or PN + GLP-2 (30 μg bid per mouse， n = 10) provided for 5 days. Compared with chow， PN reduced interleukin (IL)-4 and IL-13 lev... More
Parenteral nutrition (PN) is associated with increased infectious risks due to impaired intestinal immunity. Although glucagon-like peptide-2 (GLP-2) enhances the gut barrier function， it is uncertain whether it improves mucosal immunologic barrier function. We hypothesized that injecting the PN mouse model with GLP-2 improved innate and acquired immunity， and prevented bacterial translocation. Forty-eight hours after venous cannulation， male Institute of Cancer Research mice were randomly divided into 3 groups based on their diet: chow with saline (n = 10)， PN (n = 9)， or PN + GLP-2 (30 μg bid per mouse， n = 10) provided for 5 days. Compared with chow， PN reduced interleukin (IL)-4 and IL-13 levels (P < .05， respectively)， whereas， compared with PN alone， GLP-2 injection increased IL-4 and IL-13 levels (P < .05， respectively). Compared with chow， PN considerably suppressed， whereas GLP-2 improved， secretory phospholipase A2 and cryptdin-4 expression. PN， compared with chow， considerably decreased lysozyme and polymeric immunoglobulin receptor levels， whereas， compared with PN， GLP-2 significantly increased these protein levels (P < .01， respectively). In tissue and luminal samples， compared with chow， PN reduced secretory immunoglobulin A levels (P < .05)， whereas， compared with PN alone， GLP-2 increased secretory immunoglobulin A levels (P < .05). Functionally， more bacterial translocation was observed in the PN group compared with the chow group (P < .001)， and GLP-2 injection decreased bacterial translocation to chow levels (P < .05). In summary， GLP-2 treatment may improve intestinal innate and acquired immunity， and prevent bacterial translocation in mice on total parenteral nutrition.
