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目錄產品 » GenCRISPR™/Cas9 基因編輯相關產品 » GenCRISPR™ Cas9 Enzymes » GenCrispr NLS-Cas9-EGFP Nuclease
GenCrispr NLS-Cas9-EGFP Nuclease

In vitro DNA cleavage assay with Z03467.
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1.5% agarose gel. Input DNA is linearized pUC57 plasmid DNA. The cleavage efficiency of Z03467 is comparable to competitors.
Lane 1, marker;
Lane 2-3, DNA + gRNA + Merck-GFP;
Lane 4-5, DNA + gRNA + Z03467;
Lane 6-7, negative control.

GenCrispr NLS-Cas9-EGFP Nuclease

In vivo gene editing efficiency assay of Z03467:
Cas9 and gRNA were transfected into THP1 cells by electroporation. Cells were lysed after 48h and analyzed by T7E1 assay. The editing efficiency of Z03467 is higher than competitors.
Lane 1, marker;
Lane 2, gRNA + Z03467;
Lane 3, gRNA + Merck-GFP;
Lane 4, negative control.

GenCrispr NLS-Cas9-EGFP Nuclease

In vivo gene editing efficiency assay of Z03467:
Cas9 and gRNA were transfected into 293T cells by liposome. Cells were lysed after 48h and analyzed by T7E1 assay. The editing efficiency of Z03467 is comparable to competitors.
Lane 1, marker;
Lane 2, gRNA + Z03467;
Lane 3, gRNA + Merck-GFP;
Lane 4, negative control.

GenCrispr NLS-Cas9-EGFP Nuclease

GenCrispr NLS-Cas9-EGFP is a fusion protein developed by GenScript. It contains a nuclear localization sequence (NLS) on its N terminal and EGFP on the C terminal. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The Cas9 RNP complex can localize to the nucleus immediately upon entering the cell with the addition of a nuclear localization signal (NLS). There is no requirement for transcription and translation compared with mRNA or plasmid systems. Additionally, the Cas9 RNP complex is rapidly cleared from the cells minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). The EGFP can be used as a reporter for tracking or sorting transfected cells, which enables the possibility of enriching cell populations for desired genome edits via fluorescence activated cell sorting (FACS). It significantly reduces the labor and cost associated with single cell cloning and genotyping in genome editing applications.
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Description

GenCrispr NLS-Cas9-EGFP is a fusion protein developed by GenScript. It contains a nuclear localization sequence (NLS) on its N terminal and EGFP on the C terminal. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The Cas9 RNP complex can localize to the nucleus immediately upon entering the cell with the addition of a nuclear localization signal (NLS). There is no requirement for transcription and translation compared with mRNA or plasmid systems. Additionally, the Cas9 RNP complex is rapidly cleared from the cells minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). The EGFP can be used as a reporter for tracking or sorting transfected cells, which enables the possibility of enriching cell populations for desired genome edits via fluorescence activated cell sorting (FACS). It significantly reduces the labor and cost associated with single cell cloning and genotyping in genome editing applications.

Product Source: GenCrispr NLS-Cas9-EGFP is produced by expression from an E. coli strain.

Key Features
-DNA-free: no external DNA added to system
-High cleavage efficiency: NLS ensures the entry of Cas9 protein into nuclei
-Low off target: transient expression of Cas9 nuclease
-Time-saving: no need for transcription and translation

Applications
-Screening for highly efficient and specific targeting gRNAs by in vitro DNA cleavage.
- In vivo gene editing when combined with a specific gRNA by electroporation or injection.

Note

This is a basic protocol. The reagent concentrations, conditions, and parameters may need to be optimized.
1000 nM is equal to 190 ng/μL.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Storage & Stability GenCrispr NLS-Cas9-EGFP Nuclease is supplied with 1X storage buffer (10 mM Tris, 300 mM NaCl, 0.1 mM EDTA,1 mM DTT, 50% Glycerol PH7.4, at 25°C) and recommended to be stored at -20°C. Guaranteed stable for 18 months when properly stored.

  • GenCrispr NLS-Cas9-EGFP Nuclease
  • GenCrispr NLS-Cas9-EGFP Nuclease

    In vitro DNA cleavage assay with Z03467.
    Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1.5% agarose gel. Input DNA is linearized pUC57 plasmid DNA. The cleavage efficiency of Z03467 is comparable to competitors.
    Lane 1, marker;
    Lane 2-3, DNA + gRNA + Merck-GFP;
    Lane 4-5, DNA + gRNA + Z03467;
    Lane 6-7, negative control.

  • GenCrispr NLS-Cas9-EGFP Nuclease
  • GenCrispr NLS-Cas9-EGFP Nuclease

    In vivo gene editing efficiency assay of Z03467:
    Cas9 and gRNA were transfected into THP1 cells by electroporation. Cells were lysed after 48h and analyzed by T7E1 assay. The editing efficiency of Z03467 is higher than competitors.
    Lane 1, marker;
    Lane 2, gRNA + Z03467;
    Lane 3, gRNA + Merck-GFP;
    Lane 4, negative control.

  • GenCrispr NLS-Cas9-EGFP Nuclease
  • GenCrispr NLS-Cas9-EGFP Nuclease

    In vivo gene editing efficiency assay of Z03467:
    Cas9 and gRNA were transfected into 293T cells by liposome. Cells were lysed after 48h and analyzed by T7E1 assay. The editing efficiency of Z03467 is comparable to competitors.
    Lane 1, marker;
    Lane 2, gRNA + Z03467;
    Lane 3, gRNA + Merck-GFP;
    Lane 4, negative control.


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