Intended Use
AmMag? Ultra AT protein A magbeads
are developed and optimized
for antibody purification for high throughput and high volume applications.
Principle
The sample containing
antibody is added to the AmMag? Ultra AT protein A
magbeads and incubated on a rotor overnight or 2 hours (depending on the
concentration of samples) for the antibody to bind to the beads. The antibody
bound to the beads can be eluted off by using acidic elution facilitated by a
magnetic separation device. Magnetic separation eliminates the need for
centrifugation, minimizes the loss of sample and makes the process more user
friendly.
Material Supplied
GenScript AmMag? Ultra AT protein A magbeads are super paramagnetic
beads with diameter of 45-100 μm, covalently coated with Ultra alkaline
tolerant protein A, After treatment with 1M NaOH for 24h, the load
decreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol.
The AmMag? Ultra AT protein A
magbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. They
can be reused for up to 100 cycles. After 100 cycles, there was almost no decrease in
binding load.
Protein A, a bacterial cell
wall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s,
mainly through the Fc regions. The alkaline tolerant protein A not only keeps
the specific binding capacity to Fc region of immunoglobulin molecules, but
also tolerates alkaline conditions and can withstand rigorous cleaning
procedures.
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