圖 1. 從大腸桿菌裂解液中純化駝科 VHH#1 蛋白
泳道：
1. 蛋白Marker
2. 完整細(xì)胞裂解液產(chǎn)物
3. 流穿液
4.洗滌流穿液（第一次）
5. 洗滌流穿液（最后一次）
6. 洗脫有目的駝科VHH 單域抗體-1 的洗脫液（分子量 = 15 kDa 單體和 30 kDa 二聚體）
圖 2. 從大腸桿菌裂解液中純化駝科VHH#2 蛋白
1．蛋白Marker
2．流穿液1
3．流穿液2
4．洗脫有目的VHH2的洗脫液-1
5．洗脫有目的VHH2的洗脫液-2
6．空白洗脫液對(duì)照
7．完整細(xì)胞裂解液產(chǎn)物
MonoRab? Anti-Camelid VHH Affinity Resin
Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This single-domain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity. GenScript’s MonoRab? Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified target protein is eluted with a high recovery rate. Table 1 lists the main characteristics of GenScript’s MonoRab? Anti-Camelid VHH Affinity Resin.
| L00905 | |
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| ￥3024 | |
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| 聯(lián)系我們 | |
