| Description |
dsDNA_TRAC_EGFP HDR donor template is high purity GenWandTM closed-end linear dsDNA designed for inserting EGFP into targeted TRAC locus. The resulting EGFP expression will be placed under the endogenous TCR promoter. Advantages of GenWandTM dsDNA donor template: ? Covalently closed ends protection for better accuracy ? More suitable for scale up ? High purity and sequence verified ? Research to GMP grade Learn More>>> https://www.genscript.com/genwand-double-stranded-dna-synthesis-service.html This dsDNA HDR donor template works in combination with SafeEdit TRAC sgRNA and Cas9 protein. |
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| Sequence |
GGTTTCCTTGAGTGGCAGGCCAGGCCTGGCCGTGAACGTTCACTGAAATCATGGCCTCTT GGCCAAGATTGATAGCTTGTGCCTGTCCCTGAGTCCCAGTCCATCACGAGCAGCTGGTTT CTAAGATGCTATTTCCCGTATAAAGCATGAGACCGTGACTTGCCAGCCCCACAGAGCCCC GCCCTTGTCCATCACTGGCATCTGGACTCCAGCCTGGGTTGGGGCAAAGAGGGAAATGAG ATCATGTCCTAACCCTGATCCTCTTGTCCCACAGATATCCAGAACCCTGACCCTGCCGTG GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCT GGACCTatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgag ctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctgg cccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccac atgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgac aaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcac atggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac aagtaaTGATCATAATCAAGCCATATCACATCTGTAGAGGTTTACTTGCTTTAAAAAACC TCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTT TATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGC ATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGT CTGGATCTGCACCAGCTGAGAGACTCTAAATCCAGTGACAAGTCTGTCTGCCTATTCACC GATTTTGATTCTCAAACAAATGTGTCACAAAGTAAGGATTCTGATGTGTATATCACAGAC AAAACTGTGCTAGACATGAGGTCTATGGACTTCAAGAGCAACAGTGCTGTGGCCTGGAGC AACAAATCTGACTTTGCATGTGCAAACGCCTTCAACAACAGCATTATTCCAGAAGACACC TTCTTCCCCAGCCCAGGTAAGGGCAGCTTTGGTGCCTTCGCAGGCTGTTTCCTTGCTTCA GGAATGGCCA |
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| Quantity | 50μg | ||||||||||||||
| Storage Condition | Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles. | ||||||||||||||
| Quality Specifications |
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