| Product Description |
Recombinant HEK293T cells
stably overexpress human SARS-CoV-2 spike protein on
their surface. The surface expression of SARS-CoV-2 spike protein is
validated by FACS analysis. This stable cell line product is designed for screening
antibodies against SARS-CoV-2 spike protein, as well as for measuring binding
affinity and stability of antibody based biologics that bind with spike protein.
GenScript also offers spike protein expressing CHO-K1 stable cell line (Cat.
No. M00803) for SARS-CoV-2
study.
|
| Applications |
Cell-based binding assay; use as immunogen for
antibody development
|
| Expressed Gene |
Codon
optimized from NC_045512.2; no expressed tags
|
| Target Protein |
YP_009724390.1
|
| Host Cell |
HEK293T
|
| Size |
2 vials of frozen cells (>1X106 per
vial in 1 ml)
|
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received.
|
| Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert? PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza).
|
| Stability |
Stable through more than 15 passages with no
significant changes in assay performance or expression profile.
|
| Culture Properties |
Adherent
|
| Freeze Medium |
90% complete growth medium, 10% DMSO (Cat. No. D2650,
Sigma)
|
| Complete Growth Medium |
DMEM (Cat. No. 10569-010, Gibco), 10% FBS (Cat. No.
10099-141, Gibco)
|
| Culture Medium |
DMEM (Cat. No. 10569-010, Gibco), 10% FBS (Cat. No.
10099-141, Gibco), 1 μg/ml Puromycin (Cat. No. A11138-03, Gibco)
|
| Gene Synonyms |
S; Spike glycoprotein
|
| Background |
Severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense,
single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19).
Virus particles include the RNA genetic material and structural proteins needed
for invasion of host cells. Once inside the cell, the infecting RNA is used to
encode structural proteins that make virus particles, nonstructural proteins
that direct virus assembly, transcription, replication and host control, and
accessory proteins whose function has not been determined. The structural
proteins of SARS-CoV-2 include the envelope protein (E), spike or surface
glycoprotein (S), membrane protein (M), and the nucleocapsid protein (N). The
spike glycoprotein is found on the outside of the virus particle and gives
coronaviruses their crown-like appearance. This glycoprotein mediates
attachment of the virus particle and entry into the host cell. S protein is an
important target for vaccine development, antibody therapies and diagnostic
antigen-based tests.
|
Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on HEK293T cells. HEK293T/Spike cells (blue) and negative control HEK293T cells (grey) were probed using ACE-2 Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher).
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