| Product Description |
Recombinant HEK293 cells stably overexpress human glucagon-like peptide-1(GLP1R) on the surface and contain high levels of G
protein (Gs) to couple with the receptor in downstream signaling pathways. |
| Applications |
cAMP assay and Luciferase assay for GLP1R receptor.
|
| Expressed Gene |
Codon Optimized from NM_002062;
no expressed tags
|
| Target Protein |
NP_002053
|
| Host Cell |
HEK293
|
| Quantity |
2 vials of frozen cells (>1X106 per
vial in 1 ml)
|
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert? PLUS Mycoplasma Detection Kit (Cat. #LT07-318,
Lonza). |
| Stability |
Stable through more than 15 passages with no significant changes in
assay performance or expression profile.
|
| Culture Properties |
Adherent
|
| Freeze Medium |
90% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat.
#D2650, Sigma) |
| Complete Growth Medium |
DMEM (Cat. #10566-016, Gibco), 10% FBS |
| Culture Medium |
DMEM, 10% FBS, 400 μg/ml G418 (Cat. #10131-035,
Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen) |
| Gene Synonyms |
GLP-1
|
| Background |
GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much
lower affinity for related peptides such as the gastric inhibitory polypeptide
and glucagon. GLP1R is known to be expressed in pancreatic beta cells.
Activated GLP1R stimulates the adenylyl cyclase pathway which results in
increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a
potential target for the treatment of diabetes. GLP1R is also expressed in the
brain where it is involved in the control of appetite. Furthermore, mice which
over express GLP1R display improved memory and learning. |
Figure 1: GLP-1(7-37)-induced concentration-dependent stimulation of intracellular cAMP (Luciferase reporter) in HEK293/CRE-luc/GLP1R cells. After stimulation with GLP1R receptor agonist GLP-1(7-37), the cells are incubated with Fire-Lumi? Luciferase Assay System (L00877C, Genscript) and the relative luminescence units (RLU) were recorded on PheraStar. The RLU were plotted against the log of the cumulative doses of GLP-1(7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1(7-37) on this cell was 0.798 nM
For research use only. Not intended for human and animal therapeutic
or diagnostic use.
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