| Product Description |
Guanosine 3’, 5’‐cyclic monophosphate
(cyclic GMP; cGMP) is an important secondary messenger in signal transduction
pathways. It is a common regulator of ion channel conductance, glycogenolysis,
and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine
triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate
cyclase (GC). It activates the cGMP-dependent protein kinases which in turn
phosphorylate several downstream protein targets. cGMP has an effect on
the regulation of cAMP levels by activating or inhibiting specific
phosphodiesterases (PDEs).
GenScript
cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which
can be used for quantitative detection of cGMP in samples such as serum,
plasma, saliva, cell culture supernatant, and urine. The anti-IgG Capture Plate
is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit
Anti-cGMP Polyclonal Antibody. When free cGMP or specimen
and HRP-cGMP conjugate
are added to the well,
they compete in the solution to interact with the cGMP antibody captured
on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP
reacts with TMB Solution to develop a blue product in the solution.
The reaction is stopped by adding stop
solution and the color turns yellow which can be read at 450 nm by a Microtiter
plate reader. Becuase the concentration of cGMP-HRP is held constant in the
assay, the intensity of color is
inversely proportional to the cGMP concentration in the sample and standards.
Using the standard curve, the amount of cGMP present in the unknown samples can
be calculated by transforming its absorbance value. |
| Sensitivity |
0.11 pmol/ml |
| Detection Range |
0.3-66.7 pmol/ml |
| Test Samples |
Serum, plasma, saliva, cell culture supernatant, and urine |
| Conveniency |
All reagents and buffers for test are provided
Complete the test within 2.5 hours |
| Storage |
The unopened kit is stable for at least 12
months if stored at 2-8 °C, and the opened kit
is stable for up to 2 weeks at 2-8 °C. Do not freeze the kit. |
| Kit Contents |
| Components |
Quantity |
Part.No |
| Anti-IgG Capture Plate |
1 plate (8 wells x 12 strips) |
461-80 |
| Anti-cGMP pAb |
12 ml |
461-20 |
| HRP-cGMP |
6 ml |
461-30 |
| cGMP Standards (0, 0.3, 0.8, 2.5, 7.4, 22.2, 66.7 pmol/ml) |
1.5 ml |
461-11,12,13,14,15,16,17 |
| cGMP Standard Stock (10 |
500 μl |
461-10 |
| nmol/ml) |
|
|
| Assay Buffer A |
60 ml |
461-60 |
| Assay Buffer B |
1 ml |
461-90 |
| 20× Wash Solution |
40 ml |
B-A1-70 |
| TMB Solution |
12 ml |
B-A1-40 |
| Stop Solution |
6 ml |
B-A1-50 |
| Plate Sealer |
2 pieces |
N/A |
|
For research use only. Not intended for
human or animal clinical trials, therapeutic or diagnostic use.
