| Product Description |
Adenosine
3’, 5’ cyclic monophosphate (cAMP) is an important secondary messenger in
signal transduction pathways that follows a number of extracellular signals.
The G-Protein Coupled Receptors (GPCRs) are critical cell surface receptors
that can be activated by different ligands, such as neurotransmitters, hormones
and ions. The binding of the ligand to its receptor activates the effector
adenylyl cyclase generating cAMP product. cAMP activates or inhibits various
enzymes by promoting their phosphorylation or dephosphorylation. Its
concentration is converted from adenosine triphosphate (ATP) via adenylyl
cyclases (AC), and is inactivated by hydrolysis to 5′-AMP by the actions of
phosphodiesterases.
GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for
quantitative detection of cAMP in samples such as serum, plasma, saliva,
cell culture supernatant, and urine. The anti-IgG Capture Plate is pre-coated with a fixed amount of
Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free
cAMP or specimen and HRP-cAMP are added to the well, they compete in the
solution to interact with the cAMP
antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP
reacts with TMB Solution to develop a
blue product in the
solution. The reaction is stopped by adding stop solution and the color turns
yellow which can be read at 450 nm by a Microtiter
plate reader. Because the
concentration of cAMP-HRP is held constant in the assay, the intensity of color
is inversely proportional to the cAMP concentration in the sample and
standards. Using the standard curve, the cAMP amount
present in the unknown samples can be calculated by transforming its absorbance
value. |
| Sensitivity |
0.61 pmol/ml |
| Detection Range |
1~243 pmol/ml |
| Test Samples |
Serum, plasma, saliva, cell
culture supernatant, and urine |
| Conveniency |
All reagents and buffers for cAMP test are provided
Complete the test within 3.5 hours |
| Storage |
The
unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for
up to 2 weeks at 2-8 °C. Do not freeze the kit. |
| Kit Contents |
| Components |
Quantity |
Part.No |
| Anti-IgG Capture Plate |
1 plate (8 wells x 12 strips) |
460-80 |
| Anti-cAMP mAb |
12 ml |
460-20 |
| HRP-cAMP |
6 ml |
460-30 |
| cAMP Standards
(0, 1, 3, 9, 27, 81, 243 pmol/ml) |
1.5 ml |
460-11, 12, 13, 14, 15, 16,17 |
| cAMP Standard Stock (10 nmol/ml) |
500 μl |
460-10 |
| Assay Buffer A |
60 ml |
460-60 |
| Assay Buffer B |
1 ml |
460-90 |
| 20× Wash Solution |
40 ml |
B-A1-70 |
| TMB Solution |
12 ml |
B-A1-40 |
| Stop Solution |
6 ml |
B-A1-50 |
| Plate Sealer |
2 pieces |
N/A |
|
For
research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
