The GenScript Ni-Charged MagBeads are developed for
quick and efficient small-scale purification of polyhistidine-tagged
proteins under native or denaturing conditions.
Perform cell lysis under native or denaturing conditions. Add the cell
lysate containing your polyhistidine-tagged
proteins to the Ni-Charged MagBeads and allow the proteins bind to the MagBeads. Then
the isolated proteins can be eluted from the beads. Magnetic separation eliminates the need for
multiple tubes, minimizes the loss of sample and removes several steps of the
centrifugation process.
The GenScript Ni-Charged
MagBeads are average 40 μm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their
surfaces. They are pre-charged with nickel and ready to use for quick and
small-scale purification of polyhistidine-tagged proteins. The beads are
supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing
20% ethanol and 1 mM NiSO4. The Ni-Charged MagBeads
have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4
mL 25% slurry). |
