Figure 2: Benz-NeburaseTM Nuclease reduces viscosity of bacterial lysate by digesting nucleic acid. 2.5 U Benz-Neburase? GMP, tag free (Cat. No. Z03708) was used to treat 1 ml of bacterial lysate. Incubate at 37 °C for 30 min and then centrifuge the samples and observe the viscosity of the precipitate and the supernatant. The test results show that Benz-Neburase? GMP, tag free can greatly reduce the viscosity of bacterial lysate.
Figure 3: Benz-NeburaseTM Nuclease prevents and reduces cell clumping. The adhered cells were spread in a 24-well plate and treated with control buffer (left) and 50 U/ml Benz-Neburase? GMP, tag free (Cat. No. Z03708) (right) at 37 °C for 30 min. After the incubation, the cells were observed under a microscope. The test result show that Benz-Neburase? GMP, tag free can efficiently reduce cell clumping.
Figure 4: Benz-NeburaseTM Nuclease digests various types of nucleic acids. In a 20 μl reaction volume, 1 U of Benz-Neburase? GMP, tag free (Z03708) was used to digest different kinds of nucleic acids (PCR product, genomic DNA, plasmid DNA, and RNA) at 37 °C for 10 min. The test results show that Benz-Neburase? GMP, tag free is effective in digesting various forms of DNA and RNA.
Figure 1: Benz-NeburaseTM Nuclease removes nucleic acid residues during AAV production. 100 U Benz-Neburase? GMP, tag free (Cat. No. Z03708) was used to treat 2 ml of cell suspension at 37 °C for 60 min. Then the mix was centrifuged at 1600 g for 10 min to remove cells and cell debris, and measurements were taken for the HCD, plasmid DNA residues, and viral genome (Vg) recovery. The test results show that Benz-Neburase? GMP, tag free can remove DNA and plasmid residue in AAV virus production process more effectively than the competing product. The usage of Benz-Neburase? GMP, tag free has minimal impact on viral production.
Benz-Neburase? GMP, tag free
| Z03708 | |
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| ￥2100 | |
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| 聯(lián)系我們 | |
