A 20 μl reaction in 1 × Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR? Ultra Cas9 for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR? Ultra NLS-Cas9-Research

GenCRISPR? Ultra Cas9 is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization signal (NLS).

產(chǎn)品編號	Z03621
規(guī)格	0.5 mg:詢價 1 mg:詢價 5 mg:詢價大單詢價
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Description

The GenCRISPR? Ultra Cas9 product line provides customers with a selection of research use, GMP preclinical and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and trascprition/translational challenges.

GenCRISPR? Ultra Cas9 is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization signal (NLS).

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

產(chǎn)品概述

Key Features

High knockout efficiencies: Consistent high editing efficiency in in-vitro and in-vivo.
Tag-free: Amino acid is free from additional tagging amino acid.
Animal-free: Plant-based culture media, enzyme-free chromatographic purification.
DNA-free: No external DNA added to the system.

應(yīng)用指導(dǎo)

gRNA-dependent double-stranded DNA cleavage

產(chǎn)品屬性

Expression System	Recombinant Cas9 with an N-terminal NLS expressed by E.coli
Species	S. pyogenes
Tag	Tag-free
Molecular Weight	~160 kDa
Concentration	10 mg/mL
Active temperature	This Cas9 is active at 37 ℃.
Formulation	Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol pH 8.0 at 25 °C.
Storage & Stability	This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles.

質(zhì)控參數(shù)

Appearance	Clear, colorless liquid
Purity	≥ 95% as analyzed by SDS-PAGE ≥ 90% as analyzed by SEC-HPLC
Concentration	10 mg/ml ± 1 mg/ml
Bioactivity	≥ 90%
Residual DNase	Non-specific DNase activity
Residual RNase	Non-specific RNase activity
Endotoxin Level	≤ 10 EU/mg as analyzed by gel clotting method

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GenCRISPR? Ultra NLS-Cas9-Research

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