The primer extension assay to detect the blocking activity of Taq Antibody.
To test the blocking activity of Hot Start Taq antibody, a primer extension assay was done as follows. A pair of primers was designed with a 14 bp overlap. One is 24 bp and the other one is 41 bp. The primers were annealed and incubated with Taq DNA polymerase (lane 1-2) or Hot Start Taq DNA polymerase (lane 3-4) for 0.5 h at 65℃. The extension result was separated on an Urea PAGE gel. No primer dimers were observed with Hot Start Taq polymerase.

The PCR specificity detection of Hot Start Taq DNA polymerase.
To test the specificity of amplification, a 500 bp long HPRT fragment was amplified from human genomic DNA using Hot Start Taq DNA polymerase (lanes 1-2) and Taq DNA polymerase (lane 3-4). No non-specific bands were observed in the amplification reaction with the Hot Start Taq DNA polymerase.

Hot Start Taq DNA Polymerase

Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored.

產品編號	E00049
規格	250 U 1000 U 大單詢價
數量
價格	￥520


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Description

Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored.

產品概述

Key Features

Enhanced specificity of amplification
Reduced primer-dimer formation
Increased yield of the PCR products
Increased convenience of reactions set up at room temperature (useful in applications such as colony PCR)

產品屬性

Storage & Stability

Please store at -20°C.

應用指導

Hot Start Taq DNA Polymerase is used for PCR amplification with enhanced specificity.

圖例

The primer extension assay to detect the blocking activity of Taq Antibody.
To test the blocking activity of Hot Start Taq antibody, a primer extension assay was done as follows. A pair of primers was designed with a 14 bp overlap. One is 24 bp and the other one is 41 bp. The primers were annealed and incubated with Taq DNA polymerase (lane 1-2) or Hot Start Taq DNA polymerase (lane 3-4) for 0.5 h at 65℃. The extension result was separated on an Urea PAGE gel. No primer dimers were observed with Hot Start Taq polymerase.

The PCR specificity detection of Hot Start Taq DNA polymerase.
To test the specificity of amplification, a 500 bp long HPRT fragment was amplified from human genomic DNA using Hot Start Taq DNA polymerase (lanes 1-2) and Taq DNA polymerase (lane 3-4). No non-specific bands were observed in the amplification reaction with the Hot Start Taq DNA polymerase.

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Hot Start Taq DNA Polymerase

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