Taq DNA Polymerase, concentrated

Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency. Note: Concentrated Taq DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mix may be ordered separately.

產品編號	E00012
規格	2500 U (2500.0U/vial) 大單詢價
數量
價格	￥1200


聯系我們

Description

Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.

Note: Concentrated Taq DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mix may be ordered separately.

Note

Formulation:

Genscript Taq DNA Polymerase has been formulated using GenScript&39s proprietary technology. The enzyme can be shipped at room temperature or stored at 37°C for seven days without any significant loss of activity.

Unit Definition

one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

10 X Reaction Buffer (with Mg²⁺)

500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl₂, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 μM dNTPs.

Important: If another reaction buffers are used with Taq DNA Polymerase, Triton X-100 must be added to a final concentration of 0.1% to ensure high enzyme activity with Taq DNA Polymerase;

Concentration

Supplied in 25 units/μl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

產品概述

Key Features

Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3' end of the extension product.
High-purity: No contamination activity has been detected in standard test reactions.

產品屬性

QC

QC Tests

PCR^* performance, activity, nuclease

Lane	Taq	Amount Used
1	Leading Brand A	0.1
2		0.25
3		0.5
4	Leading Brand B	0.1
5		0.25
6		0.5
7	GenScript	0.1
8		0.25
9		0.5

Storage & Stability

Storage

Store the product at -20°C.

應用指導

Applications

The applications of Taq DNA polymerase are as follows:

PCR^*

3'A-tailing of blunt ends

Primer extension

DNA sequencing

国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

Taq DNA Polymerase, concentrated

Formulation:

Unit Definition

10 X Reaction Buffer (with Mg²⁺)

Concentration

QC Tests

Storage

Applications

資源中心

技術支持

關于我們

国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

Taq DNA Polymerase, concentrated

Formulation:

Unit Definition

10 X Reaction Buffer (with Mg2+)

Concentration

QC Tests

Storage

Applications

10 X Reaction Buffer (with Mg²⁺)