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Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's disease

Transl Neurodegener. 2020-06-01; 
Ruinan Shen, Xiaobei Zhao, Lu He, Yongbo Ding, Wei Xu, Suzhen Lin, Savannah Fang, Wanlin Yang, Kijung Sung, Brian Spencer, Robert A Rissman, Ming Lei, Jianqing Ding, Chengbiao Wu
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Polyclonal Antibody Services … University). Rabbit anti-Tau (Ser 396 ) polyclonal Antibody from GenScript (Cat# A01387). Mouse … phenylmethanesulfonyl fluoride). Protein lysates were extracted and incubated with anti-DYKDDDDK G1 affinity Resin (GenScript, L00432) and in. Get A Quote

摘要

background: In Alzheimer's Disease (AD), about one-third of the risk genes identified by GWAS encode proteins that function predominantly in the endocytic pathways. Among them, the Ras and Rab Interactor 3(RIN3) is a guanine nucleotide exchange factor (GEF) for the Rab5 small GTPase family and has been implicated to be a risk factor for both late onset AD (LOAD) and sporadic early onset AD (sEOAD). However, how RIN3 is linked to AD pathogenesis is currently undefined. methods: Quantitative PCR and immunoblotting were used to measure the RIN3 expression level in mouse brain tissues and cultured basal forebrain cholinergic neuron (BFCNs). Immunostaining was used to define subcellular localization of RIN3 and to v... More

關(guān)鍵詞

AD risk factors, Alzheimer’s disease (AD), BIN1, CD2AP, Endosomes, RIN3, Tau, Trafficking
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