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Construction and Immunogenicity Evaluation of Recombinant Influenza A Viruses Containing Chimeric Hemagglutinin Genes Derived from Genetically Divergent Influenza A H1N1 Subtype Viruses.

PLoS ONE. 2015;

McCormickKara，JiangZhiyong，ZhuLongchao，LawsonSteven R，LangenhorstRobert，RansburghRussell，BrunickColin，TracyMiranda C，HurtigHeather R，MabeeLeah M，MingoMark，LiYanhua，WebbyRichard J，HuberVictor C，Fang

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Codon Optimization	… ELRSRYWAIRTRSG). After being quadruplicated and linked with linkers (-KK- for HA91-108 and M2e, -RVKR- for NP366-374 and NP380-393), the genes were codon-optimized to mice and synthesized by GenScript (Piscataway, NJ) …	Get A Quote

摘要

Influenza A viruses cause highly contagious diseases in a variety of hosts， including humans and pigs. To develop a vaccine that can be broadly effective against genetically divergent strains of the virus， in this study we employed molecular breeding (DNA shuffling) technology to create a panel of chimeric HA genes.