G-CSF, Mouse

Granulocyte Colony-Stimulating Factor (G-CSF), also known as CSF-3 and MGI-1G, is a cytokine and hormone belonging to the IL-6 superfamily. It is expressed by monocytes, macrophages, endothelial cells, fibroblasts and bone marrow stroma. G-CSF stimulates the bone marrow to produce granulocytes and stem cells, and specifically stimulates the proliferation and differentiation of the neutrophilic granulocyte lineage. G-CSF has been used to stimulate white blood cell production after chemotherapy. It has also been used to boost the number of hematopoietic stem cells after bone marrow transplantation.

價格	￥600
產品編號	Z03163-10
規格	10 μg 50 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Mouse

Protein Construction

G-CSF (Val31-Ala208)
Accession # P09920

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.02 ng/ml, measured in a cell proliferation assay using NFS-60 cells.

Expression System

CHO

Apparent Molecular Weight

22~24 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED₅₀ < 0.02 ng/ml, measured in a cell proliferation assay using NFS-60 cells. ?

Lane 1: 3 μg of G-CSF, Mouse, non-reducing (NR)
Lane 2: 3 μg of G-CSF, Mouse, reducing (R)
> 95% as analyzed by SDS-PAGE ?

技術背景

Target Background

Synonyms

CSF3; C17orf33; CSF3OS; GCSF; colony stimulating factor 3; CSF-3; Granulocyte Colony-Stimulating Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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G-CSF, Mouse

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