KGF/FGF-7, Mouse

Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF’s roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney.

價格	￥600
產品編號	Z03154
規格	10 μg 50 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Mouse

Protein Construction

FGF-7 (Cys32-Thr194)
Accession # P36363

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 2.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 10⁵ units/mg.

Expression System

E. coli

Apparent Molecular Weight

~18.7 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED₅₀?< 2.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 10⁵?units/mg. ?

Lane 1: 2 μg of KGF/FGF-7, Mouse, reducing (R)
Lane 2: 2 μg of KGF/FGF-7, Mouse, non-reducing (NR)
> 95% as analyzed by SDS-PAGE ?

技術背景

Target Background

Synonyms

Keratinocyte Growth Factor; Fibroblast Growth Factor-7; HBGF-7; FGF7

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關文獻

1. Rongze Ma, et al. Targeting Tumor Heterogeneity by Breaking a Stem Cell and Epithelial Niche Interaction Loop. Adv Sci (Weinh). (2024-05)

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KGF/FGF-7, Mouse

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