Shh (C25II), Mouse

Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.

價格	￥500
產(chǎn)品編號	Z03050
規(guī)格	10 μg 50 μg 1 mg 大單詢價
數(shù)量

產(chǎn)品簡介圖例技術(shù)背景

產(chǎn)品簡介

Species

Mouse

Protein Construction

Shh [Cys25-Gly198 (Cys25Ile-Ile)]
Accession # Q62226

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 2.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500.0 units/mg.

Expression System

E. coli

Apparent Molecular Weight

~19.7 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED??<2.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg. ?

技術(shù)背景

Target Background

Synonyms

Hhg1; HHG-1; ShhNC; Sonic hedgehog protein N-product; Sonic hedgehog protein 19 kDa product

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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Shh (C25II), Mouse

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