GM-CSF, Mouse

Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.

價格	￥600
產品編號	Z02979
規格	10 μg 50 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Mouse

Protein Construction

GM-CSF (Ala18-Lys141)
Accession # Q14AD9

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.05 ng/ml, measured in a cell proliferation assay using mouse FDC-P1 cells, corresponding to a specific activity of > 2.0 × 10⁷ units/mg.

Expression System

CHO

Apparent Molecular Weight

15~19 kDa, on SDS-PAGE under non-reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

Lane 1: 2 μg of GM-CSF, Mouse, non-reducing (NR)
> 95% as analyzed by SDS-PAGE ?

技術背景

Target Background

Synonyms

Granulocyte Macrophage Colony Stimulating Factor; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; MGC131935; MGC138897

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關文獻

1. Yongchao Wang, et al. An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination. Nat Commun. (2021-08)

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GM-CSF, Mouse

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