G-CSF, Human

Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.

價格	￥600
產品編號	Z02974
規格	10 μg 50 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Human

Protein Construction

Expressed with an N-terminal Met.

G-CSF (Thr27-Pro200)
Accession # Q8N4W3

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.1 ng/ml, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

Expression System

E. coli

Apparent Molecular Weight

~18.8 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

2 μg of G-CSF, Human (Cat. No. Z02974) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining ?

Biological Activity
G-CSF, Human (Cat. No. Z02974) stimulates cell proliferation of M-NFS-60 cells. The ED₅₀ for this effect is typically 20-100pg/mL. ?

技術背景

Target Background

Synonyms

CSF3; C17orf33; CSF3OS; GCSF; colony stimulating factor 3; CSF-3; Granulocyte Colony-Stimulating Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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G-CSF, Human

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