TNF-α, Mouse

Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.

價格	￥1200
產品編號	Z02774-20
規格	20 μg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Mouse

Protein Construction

Expressed with an N-terminal Met.

TNF-α (Leu80-Leu235)
Accession # P06804

Purity

> 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC

Endotoxin Level

< 1 EU/μg of protein by LAL method

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg in the presence of actinomycin D.

Expression System

E. coli

Theoretical Molecular Weight

17.4 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

圖例

技術背景

Target Background

Synonyms

TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關文獻

1. Qingyun Mo, et al. An all-silk-based functional system promotes tendon regeneration by regulating the cell fate of TSPCs in an inflammatory microenvironment. Acta Biomaterialia. (2025-06)
2. Jialin Chen , et al. Controlled TPCA-1 delivery engineers a pro-tenogenic niche to initiate tendon regeneration by targeting IKKβ/NF-κB signaling. Bioact Mater. (2024-10)

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TNF-α, Mouse

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