Figure 1. GLP-1 (7-37)-induced luciferase expression in CHO-K1/CRE-CBLuc/GLP1R cells. After stimulation by agonist GLP-1 (7-37), the cells are determined with Fire-Lumi? Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC₅₀ of GLP-1 (7-37) on cells was 0.12 nM.

CHO-K1/CRE-CBLuc/GLP1R

Recombinant CHO-K1 cells stably overexpress human glucagon like peptide 1 receptor (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.

產品編號	M00969
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human glucagon like peptide 1 receptor (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham's F-12K (Kaighn's), 10% FBS, 500 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 250 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. GLP-1 (7-37)-induced luciferase expression in CHO-K1/CRE-CBLuc/GLP1R cells. After stimulation by agonist GLP-1 (7-37), the cells are determined with Fire-Lumi? Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC₅₀ of GLP-1 (7-37) on cells was 0.12 nM.

For research use only. Not intended for human and animal therapeutic or diagnostic use.

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CHO-K1/CRE-CBLuc/GLP1R

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