Figure 1. Purification of scFv containing (G₄S)₃ linker from various lysates

Lanes:
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing scFv
Lane 3: Flow through of E.coli whole cell lysate containing scFv
Lane 4: Eluate of target scFv with L01036 from E.coli whole cell lysate
Lane 5: Yeast whole cell lysate containing scFv
Lane 6: Flow through of yeast whole cell lysate containing scFv
Lane 7: Eluate of target scFv with L01036 from yeast whole cell lysate
Lane 8: Mammalian cell culture supernatant containing scFv
Lane 9: Flow through of mammalian cell culture supernatant containing scFv
Lane 10: Eluate of target scFv with L01036 from mammalian cell culture supernatant

Figure 2. Purification of bispecific antibody containing (G₄S)₃ linker from E.coli whole cell lysate

Lanes:
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing (G₄S)₃-linked BsAb
Lane 3: Flow through of E.coli whole cell lysate containing (G₄S)₃-linked BsAb
Lane 4: Eluate of target (G₄S)₃-linked BsAb with L01036 from E.coli whole cell lysate (M.W.=50 kDa)

Figure 3. Purification of protein containing (G₃S)₃ linker from E.coli whole cell lysate

Lanes:
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing (G₃S)₃-linked protein
Lane 3: Flow through of E.coli whole cell lysate containing (G₃S)₃-linked protein
Lane 4: Eluate of target (G₃S)₃-linked protein with L01036 from E.coli whole cell lysate (M.W.=50 kDa)

The target protein containing (G₄S)₃ linker was captured by GenScript Anti-GS Linker Affinity Resin FF (Cat. No. L01036). The resin was washed twice with 10 times bed volume of TBS and then eluted with 10 times bed volume of acidic eluent (0.1 M glycine HCl, pH 3.0). Subsequently, the resin was regenerated by 10 times bed volume of regeneration buffer for repeated use. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% for a total of 10 purification processes, indicating that GenScript Anti-GS Linker Affinity Resin FF is capable of multiple repeated use with relatively high consistency when eluted by acidic eluent.

Anti-GS Linker Affinity Resin FF

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

產品編號	L01036
規格	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 大單詢價
數量
價格	￥3800


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Description

A linker is an amino acid chain that connects two fused proteins, allowing for the appropriate separation of protein functional domains or the maintenance of necessary inter-domain interactions. The most widely known flexible linker is the poly-Glycine-Serine (GS) linker. The GS Linker includes various forms, such as (GS)_n, (GGS)_n, (GGGS)_n, (GGGGS)_n, etc. These GS linkers are widely used in connecting bispecific antibodies, antibody fusion proteins, single-chain antibodies, peptides and ADCs, etc.
GenScript’s Anti-GS Linker Affinity Resin FF (Cat. No. L01036) is designed for purification of proteins, antibodies and compounds containing (G_nS)_m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The GS linker fusion protein in cell lysate can specifically bind to the anti-GS linker antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified target protein is eluted with a high recovery rate. Table 1 lists the main characteristics of Anti-GS Linker Affinity Resin FF.

Table 1. Characteristics of Anti-GS Linker Affinity Resin FF

Product content	50% settled resin in TBS with 0.1% Procin300
Matrix	4% highly cross-linked agarose
Average bead size	90 μm
Ligand	Anti-GS Linker Antibody
Binding capacity	≥4 mg antibodies containing (G₄S)₃ linker per mL settled resin
Specificity	Proteins, antibodies and compounds containing (G_nS)_m linker (n≥2, m≥2), such as (G₄S)₃ linker, (G₂S)₂ linker, (G₂S)₄ linker, (G₃S)₃ linker and (G₄S)₂ linker, etc.
Storage and stability	Store at 2-8 °C for up to 12 months. Do not freeze the resin.
Resin reuse	When properly regenerated and stored according to the instructions, the resin can be reused up to 10 times without significant loss of binding capability.
Elution method	Acidic elution (pH 3.0)
Reagent compatibility	Compatible with commonly used lysis reagents. For reagents compatible with purifications, see tables 2 and 3.

Note

Limited Use Label license: This product may be the subject of one or more patents filed by GenScript Corporation.The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer should not sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. For commercial use, please contact GenScript at [email protected].

For research use only. Not intended for human and animal therapeutic or diagnostic use.

圖例

Figure 1. Purification of scFv containing (G₄S)₃ linker from various lysates

Lanes:
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing scFv
Lane 3: Flow through of E.coli whole cell lysate containing scFv
Lane 4: Eluate of target scFv with L01036 from E.coli whole cell lysate
Lane 5: Yeast whole cell lysate containing scFv
Lane 6: Flow through of yeast whole cell lysate containing scFv
Lane 7: Eluate of target scFv with L01036 from yeast whole cell lysate
Lane 8: Mammalian cell culture supernatant containing scFv
Lane 9: Flow through of mammalian cell culture supernatant containing scFv
Lane 10: Eluate of target scFv with L01036 from mammalian cell culture supernatant

Figure 2. Purification of bispecific antibody containing (G₄S)₃ linker from E.coli whole cell lysate

Lanes:
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing (G₄S)₃-linked BsAb
Lane 3: Flow through of E.coli whole cell lysate containing (G₄S)₃-linked BsAb
Lane 4: Eluate of target (G₄S)₃-linked BsAb with L01036 from E.coli whole cell lysate (M.W.=50 kDa)

Figure 3. Purification of protein containing (G₃S)₃ linker from E.coli whole cell lysate

Lanes:
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing (G₃S)₃-linked protein
Lane 3: Flow through of E.coli whole cell lysate containing (G₃S)₃-linked protein
Lane 4: Eluate of target (G₃S)₃-linked protein with L01036 from E.coli whole cell lysate (M.W.=50 kDa)

The target protein containing (G₄S)₃ linker was captured by GenScript Anti-GS Linker Affinity Resin FF (Cat. No. L01036). The resin was washed twice with 10 times bed volume of TBS and then eluted with 10 times bed volume of acidic eluent (0.1 M glycine HCl, pH 3.0). Subsequently, the resin was regenerated by 10 times bed volume of regeneration buffer for repeated use. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% for a total of 10 purification processes, indicating that GenScript Anti-GS Linker Affinity Resin FF is capable of multiple repeated use with relatively high consistency when eluted by acidic eluent.

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Anti-GS Linker Affinity Resin FF

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