FACS analysis of PD-1 expression in Daudi/PD-1 cells.
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| Product Description |
Recombinant Daudi cells stably overexpress human programmed cell death
protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by
FACS analysis. |
| Culture Properties | Suspension |
| Stability |
Stable through more than 15 passages with no significant changes in assay
performance or expression profile. |
| Size |
Two vials of frozen
cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium |
RPMI 1640 Medium, 20% FBS, 0.5 μg/ml Puromycin (Cat. No. A11138-03,
Gibco), 1% Sodium Pyruvate (Cat. No. 11360-070, Gibco) |
| Complete Growth Medium | RPMI 1640 medium, 20% FBS |
| Freeze Medium-DATA | 90% FBS (Cat. No. 10099-141, Gibco), 5% RPMI 1640 medium (Cat. No. 22400, Gibco), 5% DMSO (Cat. No. D2650, Sigma) |
FACS analysis of PD-1 expression in Daudi/PD-1 cells.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
