Figure 1: FACS analysis of cell surface expression of human CD32A 131His on CHO-K1 cells.
CHO-K1/human CD32A 131His cells (blue) and negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).

CHO-K1/human CD32A 131His Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa/CD32A 131His) on the surface. The surface expression of CD32A 131His is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131His. GenScript also offers CD32A 131Arg stable cell line (Cat. No. M00887) for FcγRIIa polymorphism study.

產(chǎn)品編號	M00598
規(guī)格	2 vials 大單詢價
數(shù)量
價格	詢價


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產(chǎn)品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa/CD32A 131His) on the surface. The surface expression of CD32A 131His is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131His. GenScript also offers CD32A 131Arg stable cell line (Cat. No. M00887) for FcγRIIa polymorphism study.
Culture Properties	Adherent
Stability	Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1: FACS analysis of cell surface expression of human CD32A 131His on CHO-K1 cells.
CHO-K1/human CD32A 131His cells (blue) and negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/human CD32A 131His Stable Cell Line

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