Product Description |
Recombinant CHO-K1 cells stably overexpress cynomolgus C-type lectin domain family 4 member C (BDCA2) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of BDCA2 is validated by FACS analysis. |
Culture Properties |
Adherent |
Stability |
Stable through more than 16 passages without significant changes in assay performance or expression profile. |
Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Techonologies) |
Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Techonologies), 10% FBS (Cat. No. 10099-141, Life Techonologies) |
Freeze Medium-DATA |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |

Figure 1. FACS analysis of cynomolgus BDCA2 expression in CHO-K1/cyno BDCA2/cyno FcER1G cells. The CHO-K1/cyno BDCA2/cyno FcER1G cells (blue) and the negative control CHO-K1 cells (red) were probed using PE conjugated Anti-BDCA2 (Cat. No. 130-090-511, Miltenyi).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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