Figure 1: FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells.
The CHO-K1/CD64 cells (blue) and the negative control CHO-K1 cells (red) were stained with PE Mouse anti-Human CD64 (Cat. No. 558592, BD Bioscience).

CHO-K1/CD64 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI, CD64) on the cell surface, containing one alpha chain and two gamma chains. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.

產品編號	M00588
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI, CD64) on the cell surface, containing one alpha chain and two gamma chains. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.
Culture Properties	Adherent
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 300 μg/ml Hygromycin B (Cat. No.10687010, Invitrogen), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco)
Complete Growth Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Freeze Medium-DATA	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1: FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells.
The CHO-K1/CD64 cells (blue) and the negative control CHO-K1 cells (red) were stained with PE Mouse anti-Human CD64 (Cat. No. 558592, BD Bioscience).

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CHO-K1/CD64 Stable Cell Line

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